Aminotransferase Alanine landa - uphawu kweenkqubo ezithintela ukosuleleka kunye sisiba mandundu kwizigqeba

aminotransferase ngenxa kokuthutha ukusuka NH2-amaqela kunye γ-acid acid ngegazi le acceptor - α-acid acid ngaphandle ukuyilwa eliphakathi ze ammonium. Oko kukuthi, transamination (transamination: transfer ingathityazwa lwamaqela acid ukuba nee acid) - inkqubo wavula zizazinzulu Russian adumileyo kunye Kritsman Braunstein.

acid (aspartic, glutamic kunye alanine) ngexesha transamination iguqulelwa ehambelana α-nee-asidi, ezo amacandelo zomjikelo acid tricarboxylic. Ngexesha igcwala abo kumjikelo Krebs, ukuba umthombo wamandla.

Transamination kubalulekile ekunikeni umjikelo urea aspartate.

Ngaphandle kwento yokuba reactions transamination ukuba lungakhuseleki kunye acid enuka: phenylalanine, tyrosine kunye tryptophan - isicelo eluncedo amayeza ufumene inkcazo yomsebenzi of aspartate kunye aminotransferase alanine. Ezi enzyme ezisasazwa kwi nezihlunu umzimba womntu.

aminotransferase Alanine elandileyo iiseli ezibomvu egazini kunye eyavela ngokuphindwe kathandathu eliphezulu xa kuthelekiswa ukuba i-serum egazini. Ukufumaneka eliphezulu enzyme ezisisigxina kwizihlunu esibindini.

Alanine aminotransferase norm alufani ukuba ancedisana umzimba womntu. Umzekelo umsebenzi enzyme izihlunu kwentliziyo ngu 7.1 (E * 10-3 g izihlunu homogenate), esibindini - 44, izihlunu skeletal - 4,8, zezintso - 19, udakada - 1.2, 0.7, and umphunga serum - 0,016. iindlela ezikhoyo ukuqingqwa komsebenzi aminotransferase kwi serum yahlulwe ngamaqela amabini: sokugcina (colorimetric, esebenzisa eqhelekileyo, hayi mayixhotyiswe lobushushu elawulwa Cuvette photometric izixhobo) nawentshukumo (spectrophotometric).

B6 kuyinto vitamin eziqhelekileyo iindidi zokutya. Nangona kunjalo, kufuneka kukhunjulwe ukuba ngexesha lenkqubo yokupheka (obrobka thermal) i-vitamin itshabalele. Pyridoxine i cofactor kwi enqanda ezininzi. Ukugxila pirodoksin-5-phosphate egazini kuyancipha kunye nobudala. Ngoko ke, lo msebenzi kule enzyme alanine aminotransferase, sehla abantu abadala ngaphezu kweminyaka 64 (30%) ngaphandle kwezo minyaka 46-63 abaneminyaka. Uyondelelwano lwee- vitamin B6 kwi serum igazi oonobangela alanine aminotransferase.

Ukuze ubone sokusebenza kwesibindi isoloko kuvavanywa kumanqanaba aminotransferase kwigazi isigulana. Oku kubangelwa ikakhulu yokuba utshintsho kumsebenzi wabo kwenzeka ngokukhawuleza kakhulu kunezinye iparameters elebhu. Wokukhutshwa eluhlwini (uphume) le-enzyme zesibindi egazini na okwalathisayo ukuba cytolysis - iseli ukutshatyalaliswa okanye ukuphazamiseka permeability lwabo ndizayo. Xa kuhlalutywa utshintsho kumsebenzi enqanda Kubalulekile ukuseka indawo emzimbeni. Kufuneka wazi ukuba umsebenzi wabo iiseli igazi umzimba usempilweni, kwasendaweni subcellular futhi umzimba. I-enzayim ye xi - alanine banda, ingakumbi iiseli zesibindi, ke ngoko, nokuba umonakalo kancinane kwabo kuphakamisa ukwanda umsebenzi enzyme egazini. Ngenxa umsebenzi enzyme ukufumanisa izifo kwesibindi (hepatitis, steatosis).

aminotransferase Alanine landa necrosis kwesibindi, kwakunye parenchymal hepatitis. Le xi phantsi enzyme abhaliswe kohlobo kunye muscular. Kufuneka ke ukuba ukwanda umsebenzi aminotransferase kwi serum uqala kwiintsuku 3-8 phambi kokuqala iimpawu eziphambili kwezonyango sifo yaye ifikelela ubuninzi ngemihla ekuqaleni yophuhliso inkqubo yezifo.

Ngenxa yoko, aminotransferase alanine ukwanda: kwesibindi, nenyongo, umhlaza wesibindi, iposi engabalulekanga, ukusilela tye na intliziyo, hypoxia, ukothuka, myositis, myocarditis, steatosis, pancreatin, butywala esinganyangekiyo.