Ngayiphi injongo isampuli ebanjwe Coombs?

Coombs - uvavanyo kwilebhu ethile evumela ukumisela antibodies kwi plasma okanye elibomvu legazi komhlaba. Le nkqubo ivumela ukuxilonga omzimba anaemia hemolytic, kuquka usana, kwakunye ukuchonga ukusabela hemolytic. Coombs aza kusetyenziswa uphando kwinzululwazi basenkundleni kwaye yemfuza ukuba ukujonga awakwazi erythrocyte. Ukuthobela yonke imithetho azihlole yenza kube lula ukufumana iziphumo inokuthenjwa.

Injongo test antiglobulin

Direct uvavanyo Coombs uyakwazi ukubona izilwa-anti-erythrocytic, zona zimiliselwe erythrocytes. indlela entle xa isifundo efanayo salatha kuphuhliso samathambo anemia hemolytic. Kufuneka kuqatshelwe ukuba isiphumo esithi ayikho ngaphandle ubukho isifo samathambo, ekubeni izilwa afunyanwa rhoqo kwi uhlobo ezamahala, ngamanye kuba akukho kuhlobana erythrocytes. Kwiimeko ezinjalo, kucetyiswa ukuba enze uvavanyo ngqo Coombs, nto leyo eza kuqinisekisa arhente abazilawulayo serum.

Njani na uhlalutyo?

W Izikhwebu igazi lwemithambo ze isigulane lwenziwa ungatyanga kusasa, nangona ukuba naziphi izinto ezibalulekileyo eziphembelela umphumo lokugqibela kolu vavanyo, noko kutyhilwa. izinto Store kuvunyelwe ezithathwe °-2 ukuya ku-8 C esingekho ngaphezu kweentsuku ezisixhenxe. Nezalathisi zesi sifundo kangangoko ngqo kangangoko kunokwenzeka, lonke igazi ngethuba leeyure ezimbini zokuqala ukuba kusiwa elebhu. Ngokunqwenelekayo Coombs isampuli kufuneka sibonise isiphumo esithi, ebonisa ukungabikho utshintsho hemolytic emzimbeni.

ziqonde iitotali

Coombs na indlela uphando kunokuba nzima, nto leyo endinga ngenyameko yaye ichanile. Xa usebenzisa uvavanyo olo nazo ezinye iingxaki sizibandakanya ukutyhilwa olungachanekanga iziphumo zokugqibela ngenxa yokuba imbonakaliso ezibuthathaka basabele kahle. Kufuneka kuqatshelwe ukuba uhlalutyo unreliability - ezizezi HIV Coombs - inokuba njengesiphumo kakuhle zoqhagamshelwano ukuhlamba erythrocytes ne okunamafutha
amacandelo angaphezulu neutralization antiglobulin nezinto serum. Enye ilahleko yale ndlela xenga izixhobo zokufunda ethathwe zidipozithwe leyo eye iimpawu ezithile.

Unobangela japan ezimbi lobuxoki-kunokuba ngokugqithisileyo behlunguzela ekunqunyanyisweni erythrocytes in resuspension. iziphumo ezitenxileyo Kwakhona ngenxa yobukho ubumdaka anticomplement unalo zona adsorbed phezu komhlaba ngexesha bafukanywe le erythrocytes test, lonto iyakuvelisa ukuvela iziphumo ezidibanisayo. Ukuba uhlamba ngenyameko iisampulu zovavanyo iliso iimeko reaction, aba neengxaki ngokulula lupheliswe, nto leyo eza kwandisa amathuba okufumana ezi zalathisi oluthembekileyo Coombs test.