Lwegazi.

Group igazi lomntu esizalwa into kwaye emiselwe kweejini yabazali. Kukho iindlela ezininzi yamaqela igazi. Ethandwa kakhulu ABO, nto leyo umiselwa zofuzo esihambelanayo.

Igazi yeqoqo kuyimfuneko ngaso nasiphi ukutofelwa (wapakisha iiseli ezibomvu plasma), kwakunye imisebenzi yotyando. Nayiphi manipulation kwinyama omzimba womntu ifuna ukuthobela antigenic lomntu, nto leyo kumiselwa inkqubo iqela AB0.

Namhlanje, kukho iindawo ezimbini eziphambili (indlela) apho ukuzimisela leqela kwegazi. Zombini ezi uthembeke. Wokuqala kwaye elula - igazi iqela yokusabela izogemagglyutinatsii. Second - Cross indlela (agglutinogens bayithwala ukuchonga indlela yokuqala kunye definition agglutinin usebenzisa erythrocyte nokumiswa). Ukwehluka indlela yokuqala - iqela Colyclons igazi (nshita indlela zitofu standard lakufumana kungcoliseko Colyclons HIV kunye hepatitis).

iimvavanyo Ukusekwa kunye sera izogemagglyutiniruyuschimi

Kwezi sera eziqulathwe agglutinins (antibodies) ukuya kumaqela amane, nto leyo ogqitywe umsebenzi titer.

Xa kuxutywa le serum kunye sera isigulane standard ukumisela iqela agglutination waphawula xa (positive abasabela - lib erythrocytes kulandele flocculation, decolorized kunye serum). Ukusabela okunge- kuboniswa umbala uniform opinki ngaphandle flocculation.

nokutolikwa iziphumo

Ukuba isicwangciso reaction 3 serum ukunika impendulo elibi, ngoko - iqela igazi O (I).

Kuba O HIV (I) kunye In (III) kunye navumelanayo A (II) - A lwegazi (II).

Ukuba serum O (I) A kunye (II) - HIV, kwaye (II) - okubi, iqela In (III).

Xa zonke zontathu sera ukunika indlela entle, iqela igazi AB (IV). Kwimeko enjalo, kuyimfuneko ukuba aqhube uphononongo ezongezelelweyo kunye serum igazi AB (IV) qela. agglutination okugwenxa kungqina AB (IV) lwegazi.

Nge Colyclons group igazi kumiselwa ngendlela efanayo. Umahluko omkhulu ukusuka poliagglyutinatsii Colyclons zitofu mgangatho ukungabikho epheleleyo erythrocytes ngenxa reactions non-ngqo. Enye inkalo ekhethekileyo tsoliklonalnyh abasabela kumaqela isifo AB0 - avidity eliphezulu (umz ezibonwayo ibinzana agglutination). Xa kuthelekiswa Colyclons standard sera avidity aphezulu kakhulu, nto leyo eyandisa ukuchana yeseti iisampuli.

Indlela yokuqingqa iqela igazi yakhiwe wanciphiswa ekuqulunqeni ngayo agglutination ethile. On planchette (okanye ipleyiti) zisetyenziswe amaqula Colyclons ezizodwa okanye serum umgangatho, ndaza ke elowo nalowo kubo idityaniswa amathontsi ambalwa igazi zivavanywayo. Reaction ibanjiwe phakathi kwemizuzu emibini okanye emithathu kunye planchettes behlunguzela (amacwecwe) kunye wezenzi. Njengoko kukhankanyiwe ngasentla - iifom kukubola enkulu eqaqambileyo ebomvu ukubonisa iziphumo ezihle, okt phambi awakwazi ukuba izilwa-serum eqhelekileyo kweli qela gazi.

Ngoko ke, ukuzimisela amaqela igazi ziqondile kwaye yokusabela kuqhutywa kwiiyantlukwano ezimbini ezisisiseko ze-buciko. Abo kakhulu ezilukhuni ezifundisayo kakhulu kwaye athembekile. Ukubaluleka lwegazi bene ngokuchanekileyo liphezulu kakhulu, njengoko na ukuba zithathelwe ingqalelo ngexesha igazi ezahlukeneyo kunye nokufakelwa. Kungenxa yoko le nto phambi kokuba benziwe naluphi utyando nokusindlekwa neesampule ukuqinisekisa iqela igazi yesigulana.